Saccharification of pineapple peelings through dilute acid hydrolysis

According to the Worldwatch Institute, between andthe population is more than tripled and the energy consumed rose more than 12 percent. IJERTV4IS Because of the energy crisis inthe development in using renewable energy sources evolved and ethanol has been one of the focuses in researches.

The fermentation broth subjected to pH 5. Based on this correlation, the amount of cell that was used in fermentation was 5. It has been a growing interest for many years because of its alternative green energy sources, consequently minimizing greenhouse gas GHG emission and finally help alleviate the rise of fuel prices [].

Lignocellulosic biomass has the most expensive pre-treatment of all the types of biomass.

Bioethanol Production from Pineapple (Ananas ...

Recent advances in plant genomics have led to large and diverse genome libraries of plant species that can improve our understanding of how individual plant species perform in ethanol-production processes to help guide future development of feedstocks with potentially advantageous characteristics for cellulosic ethanol production.

The hydrolysate was neutralized with 1. Alcohol was determined using Gas Chromatography. Pineapple was initially used up only as a fresh fruit.

Conventional laboratory pretreatment, carried out in tubes, mixed reactors or steam guns, with subsequent enzymatic hydrolysis, requires larger amounts of biomass materials than may be available without sacrificing the plants when screening large numbers of biomass candidates.

The yeast was asceptically transferred to 20 mL potato dextrose broth and placed in a shaker at a rate of 30rpm for 18 hours. The result of standardization is presented as Figure 1.

Example of this is sugar cane. It was washed and chopped into smaller pieces and then subjected to size reduction using a blender. The extract was filtered in a 1L beaker using cheesecloth. In this study, the effect of fermentation pH was investigated.

Dilute acid pretreatment, enzymatic saccharification, and fermentation of rice hulls to ethanol.

Therefore, development of screening tools for HT pretreatment and enzymatic hydrolysis to identify biomass variants with reduced recalcitrance has recently attracted interest [ 3 - 5 ]. These samples were subjected to high temperature short time HTST pasteurization after 48 hours for the consequent ethanol purity testing.

The aim of this study is to investigate the potential of Pineapple peelings as an alternative source of bioethanol using Saccharomyces cerevisiae as yeast. Sugar-containing biomass can be readily fermented without undergoing chemical or biological pre-treatments.

It also does not simulate the most attractive commercial operations, for which it is preferable to avoid separation of solids from liquids, in order to reduce capital costs and opportunities for contamination.

With the help of the developing technology and promising researches, the fruit is now prepared and consumed in different forms. Results For hydrothermal pretreatment at solids concentrations of 0. Published online Jul Unlike wood residue and straw, the low lignin content of pineapple may be related to its soft structure [8].

In order to obtain the growth rate of specific yeast, standardization must be done.

The calcium hydroxide was also simultaneously autoclaved at the same conditions.Hydrolysis of Polysaccharides with 77% Sulfuric Acid for Quantitative Saccharification quantitative saccharification, acid hydrolysis, polysaccharides, wood, pulp, HPLC composition to wood or pulp samples were run through.

After standardization, the pineapple peelings were subjected to a dilute-acid pre-treatment using 5% (v/v) sulphuric acid for 2 hours at 90oC yielding % (w/v) reducing sugar. The resulting solutions after hydrolysis are then subjected to fermentation at different pH readings.

This study entitled “Saccharification of Pineapple Ananas comosus peelings through Dilute Acid Hydrolysis” was show more content A clear aqueous solution of carbohydrates to be analyzed is placed in a test tube, then phenol and sulfuric acid are solution turns a yellow- orange color as a result of the interaction between.

The objective of this work is to identify the optimum conditions for the dilute acid pretreatment of eucalyptus, which was evaluated by the xylose plus glucose, both from dilute sulphuric acid pretreatment and enzymatic hydrolysis. Results. For hydrothermal pretreatment at solids concentrations of to 2%, high enzyme protein loadings of about mg/g of substrate (glucan plus xylan) in the original poplar wood achieved glucose and xylose yields for co-hydrolysis that were comparable with those for washed solids.

Hydrolysis of orange peel with very dilute (pH=) sulfuric acid leads to a high level of sol- ubilization of orange peel solids and, due to lower acid consumption and lower destruction of fructose, is preferable over treatment with more concentrated acid solutions.

Saccharification of pineapple peelings through dilute acid hydrolysis
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